Author

D. E. Brackney

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 7 Evolutionary Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 7 Organism:Arabidopsis· 4 Research Focus:host-pathogen & immune response· 4 Assay:bulk RNA-seq· 2 Research Focus:ecology & biodiversity· 2 Assay:metagenomics· 2 Approach:statistical model· 2 Research Focus:cell type annotation· 1

Frequent Co-Authors

B. Steven· 3 shared J. Hyde· 2 shared 2022 UW-Madison Capstone in Microbiology Students· 1 shared A. Bransfield· 1 shared A. Casanovas-Massana· 1 shared A. F. Hoque· 1 shared A. Foo· 1 shared A. Iwasaki· 1 shared

Activity by Year

2018-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 10 mutual co-author links

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3 shared papersMicrobiology

B. Steven

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates -- MosAIC (Mosquito-Associated Isolate Collection)

A. Foo, L. E. Brettell, H. L. Nichols, 2022 UW-Madison Capstone in Microbiology Students, M. Medina Munoz, J. Lysne, V. Dhokiya, A. F. Hoque, D. E. Brackney, E. P. Caragata, M. Hutchinson, M. Jacobs-Lorena, D. J. Lampe, E. Martin, C. Valiente Moro, , E. Heinz

The paper establishes a high-quality bacterial isolate collection (MosAIC) from mosquitoes, providing genomic data and phylogenomic analysis to study mosquito-associated bacteria.

PLOS Biology · Nov 15, 2024Peer-reviewedMicrobiology

Mosquito immune cells enhance dengue and Zika virus dissemination in Aedes aegypti

D. R. Hall, R. M. Johnson, H. Kwon, Z. Ferdous, S. V. Laredo-Tiscareno, B. J. Blitvich, D. E. Brackney, R. C. Smith

This study shows mosquito immune cells help spread dengue and Zika viruses in Aedes aegypti, supporting a 'trojan horse' model of virus dissemination.

bioRxiv · Apr 3, 2024 · PreprintMicrobiology

Three Species of Axenic Mosquito Larvae Recruit a Shared Core of Bacteria in a Common Garden Experiment

J. Hyde, D. E. Brackney, B. Steven

This study generates three axenic mosquito species and shows they share a core microbiome but have unique structures, with co-rearing leading to microbiome convergence.

bioRxiv · Mar 23, 2023Microbiology

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR

E. Kudo, B. Israelow, C. Vogels, P. Lu, A. L. Wyllie, M. Tokuyama, A. Venkataraman, D. E. Brackney, I. Ott, M. Petrone, R. Earnest, S. Lapidus, C. Muenker, A. Moore, A. Casanovas-Massana, , A. Iwasaki

This paper develops a multiplex RT-qPCR assay for SARS-CoV-2 detection that uses existing primer/probe sets and reduces reagents, costs, time, and labor compared to singleplex assays.

bioRxiv · Jun 16, 2020Microbiology

A limited role for autophagy during arbovirus infection of mosquito cells

D. E. Brackney, M. A. Correa

This study investigates autophagy's role in arbovirus infection of mosquito cells, finding limited involvement and unexpected effects of autophagy modulators on viral replication.

bioRxiv · Sep 12, 2019Microbiology

Antibiotic resistant bacteria and commensal fungi are common and conserved in the mosquito microbiome

J. Hyde, C. Gorham, D. E. Brackney, B. Steven

This study identifies antibiotic-resistant bacteria and fungi in mosquito microbiomes, showing their prevalence and conservation across species.

bioRxiv · Jun 13, 2019Microbiology

An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar

N. D. Grubaugh, K. Gangavarapu, J. Quick, N. L. Matteson, J. Goes De Jesus, B. J. Main, A. L. Tan, L. M. Paul, D. E. Brackney, S. Grewal, N. Gurfield, K. K. Van Rompay, S. Isern, S. F. Michael, L. L. Coffey, , K. G. Andersen

The paper evaluates PrimalSeq for measuring intrahost virus diversity, showing how factors like sequencing coverage and primer mismatches affect accuracy.

bioRxiv · Aug 5, 2018Evolutionary Biology

SUCCESSIVE BLOODMEALS ENHANCE VIRUS DISSEMINATION WITHIN MOSQUITOES AND INCREASE TRANSMISSION POTENTIAL

P. M. Armstrong, H. Ehrlich, A. Bransfield, J. L. Warren, V. E. Pitzer, D. E. Brackney

The paper shows that successive bloodmeals shorten the extrinsic incubation period of viruses in mosquitoes, increasing transmission potential and explaining epidemic dynamics.

bioRxiv · Jan 10, 2018Microbiology