The paper investigates how soluble MAC (sMAC) is formed during bacterial infections, finding that MAC-resistant bacteria convert C5 into C5b more efficiently and release sMAC, which can lyse bystander erythrocytes.

DOI 10.7554/elife.77503

humanproteomics

𝕏 PostBluesky

MicrobiologybioRxivMay 12, 2021

Polymerization of C9 enhances bacterial cell envelope damage and killing by membrane attack complex pores

D. J. Doorduijn, D. A. C. Heesterbeek, M. Ruyken…·B. W. Bardoel

This study shows that C9 polymerization enhances MAC pore-mediated bacterial killing and is impaired in complement-resistant strains.

DOI 10.1371/journal.ppat.1010051

E. coliexperimental assay

𝕏 PostBluesky

MicrobiologybioRxivMay 24, 2020

In vitro evolution of colistin resistance in the Klebsiella pneumoniae complex follows multiple evolutionary trajectories with variable effects on fitness and virulence characteristics.

A. B. Janssen, D. J. Doorduijn, G. Mills…·W. van Schaik

This study shows that Klebsiella pneumoniae complex strains can develop colistin resistance through multiple evolutionary paths, with variable effects on fitness and virulence.

DOI 10.1128/aac.01958-20

humanwhole-genome sequencing

𝕏 PostBluesky

ImmunologybioRxivDec 17, 2019

Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7

D. J. Doorduijn, B. W. Bardoel, D. A. Heesterbeek…·S. H. Rooijakkers

This study shows that direct anchoring of C5b-7 is essential for MAC pore formation and bacterial killing, and bacteria can evade this by interfering with anchoring.

DOI 10.1371/journal.ppat.1008606

E. coliexperimental assay

𝕏 PostBluesky