B. Hoogenboom

Lipid specificity of the immune effector perforin

This study investigates how lipid composition affects perforin function using atomic force microscopy on model membranes.

Physical modelling of multivalent interactions in the nuclear pore complex

This study develops computational models to analyze the role of heterogeneity in FG Nups and NTRs, finding minor effects on binding equilibria but significant impacts on kinetics and multivalency.

TopoStats - a program for automated tracing of biomolecules from AFM images

TopoStats automatically traces biomolecules in AFM images with high accuracy, identifying 90% of molecules and assemblies without prior processing.

Structural basis for tuning activity and membrane specificity of bacterial cytolysins

This study investigates how bacterial cytolysins use cholesterol to selectively lyse cells by analyzing structural changes in intermedilysin bound to CD59.

Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7

This study shows that direct anchoring of C5b-7 is essential for MAC pore formation and bacterial killing, and bacteria can evade this by interfering with anchoring.

Intrinsically disordered nuclear pore proteins show ideal-polymer morphologies and dynamics

This study shows FG nups in the nuclear pore complex exhibit ideal-polymer behavior, balancing repulsive and attractive interactions to enable both cohesion and dynamic transport.

Imaging live bacteria at the nanoscale: comparison of immobilisation strategies

The paper compares immobilisation strategies for live Escherichia coli using AFM and fluorescence microscopy to assess adsorption, viability, and compatibility with imaging.

PEGylated surfaces for the study of DNA-protein interactions by atomic force microscopy

This paper demonstrates the use of PEGylated surfaces to selectively adsorb DNA on mica for AFM imaging, enabling clear visualization of DNA-protein interactions in high-protein concentration solutions.

Quantification of biomolecular dynamics inside real and synthetic nuclear pore complexes using time-resolved atomic force microscopy

The paper uses AFM to study biomolecular dynamics in real and synthetic nuclear pore complexes, revealing slow collective transitions in mimetic NPCs due to FG-nup interactions.