This paper reports the 2.8Å X-ray structure of zoliflodacin bound to Staphylococcus aureus DNA gyrase, showing its mechanism of DNA cleavage inhibition and improved antibacterial activity.

DOI 10.1101/2022.11.30.518515

protein structureexperimental assay

MicrobiologybioRxivMar 5, 2021

Exploration of polyyne biosynthetic gene cluster diversity in bacteria leads to the discovery of the Pseudomonas polyyne protegencin

A. J. Mullins, G. Webster, H. J. Kim…·E. Mahenthiralingam

The paper explores polyyne biosynthetic gene clusters in bacteria, identifies a conserved seven-gene cassette in Pseudomonas, and discovers the metabolite protegencin.

DOI 10.1128/mbio.00715-21

bacteria (other)whole-genome sequencing

MicrobiologybioRxivPreprintJun 16, 2020

Discovery and biosynthesis of gladiochelins: unusual lipodepsipeptide siderophores from Burkholderia gladioli

Y. Dashti, I. Nakou, A. Mullins…·G. Challis

This study identifies two novel lipodepsipeptide siderophores in Burkholderia gladioli, showing they bind ferric iron and are involved in iron acquisition.

DOI 10.1101/2020.06.16.153940

bacteria (other)experimental assay

MicrobiologybioRxivApr 9, 2020

Kill and cure: genomic phylogeny and bioactivity of a diverse collection of Burkholderia gladioli bacteria capable of pathogenic and beneficial lifestyles

C. Jones, G. Webster, A. J. Mullins…·E. Mahenthiralingam

This study examines the genomic phylogeny and bioactivity of 206 Burkholderia gladioli strains, identifying toxin-producing clades and potential therapeutic strategies for CF-related infections.

DOI 10.1099/mgen.0.000515

humanDNA methylation