Gene editing of CD3 epsilon gene to redirect regulatory T cells for adoptive T cell transfer
W. Du, F. Noyan, O. McCallion, V. Drosdek, J. Kath, V. Glaser, C. Fuster-Garcia, M. Yang, M. Stein, O. Weber, J. K. Polansky, T. Cathomen, E. Jaeckel, J. Hester, F. Issa, , D. L. Wagner
This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.
bioRxiv · Mar 18, 2024 · PreprintImmunology