T. Cathomen

Gene editing of CD3 epsilon gene to redirect regulatory T cells for adoptive T cell transfer

This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.

Integration of ζ-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

This paper develops a method to integrate ζ-deficient CARs into the CD3ζ gene to create functional CAR-T and CAR-NK cells with improved safety and efficacy for off-the-shelf therapies.

Epitope Engineered Human Haematopoietic Stem Cells are Shielded from CD123-targeted Immunotherapy

This paper shows that epitope-engineered human hematopoietic stem cells are protected from CD123-targeted immunotherapy while maintaining functionality, offering a strategy for tumor-selective therapy.

Efficient in vivo genome editing mediated by stem cells-derived extracellular vesicles carrying designer nucleases

This study demonstrates that stem cell-derived extracellular vesicles can deliver designer nucleases in vivo, achieving up to 50% gene knockout in the liver.

HIV-1 promoter is gradually silenced when integrated into BACH2

This study shows that HIV-1 promoters integrated into BACH2 are gradually silenced over time in Jurkat T-cells, without affecting BACH2 expression, potentially contributing to HIV-1 persistence.