H.-D. Volk

Gene editing of CD3 epsilon gene to redirect regulatory T cells for adoptive T cell transfer

This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.

Single cell analysis reveals CD45RClow/-TNFR2+CD29lowCD8+ Tregs with superior activity

The paper identifies and characterizes three subsets of human CD8+ regulatory T cells using single-cell CITE-seq and VDJ sequencing, highlighting a subset with superior suppressive activity.

Integration of ζ-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

This paper develops a method to integrate ζ-deficient CARs into the CD3ζ gene to create functional CAR-T and CAR-NK cells with improved safety and efficacy for off-the-shelf therapies.

Matching or genetic engineering of HLA Class I and II facilitates successful allogeneic 'off-the-shelf' regulatory T cell therapy

This study shows that matching or engineering HLA-I and HLA-II improves the efficacy of allogeneic regulatory T cells for immunoregulation.

Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells

This paper combines CRISPR nucleases to simultaneously knock-in and base edit CAR T cells, reducing translocations to 1.5% and improving safety.

Human iPSC-derived renal cells change their immunogenic properties during maturation: Implications for regenerative therapies

This study shows that hiPSC-derived renal cells become less immunogenic as they mature, with implications for improving immune tolerance in regenerative therapies.

Fast, efficient and virus-free generation of TRAC-replaced CAR T cells

This paper presents a virus-free method to generate CAR T cells by inserting CARs into the TRAC locus using CRISPR-Cas and HDR, achieving up to 68% TCR-to-CAR replacement.

Extra-hematopoietic immunomodulatory role of the SCID-susceptibility gene DOCK-2 identified by stepwise maturation of human iPSCs into clonogenic mesodermal stromal progenitors

This study identifies DOCK-2's role in stromal immunomodulation through iPSC differentiation and CRISPR knockout, showing reduced immunomodulatory capacity in DOCK-2-deficient cells.

High prevalence of S. pyogenes Cas9-specific T cell sensitization within the adult human population - A balanced effector/regulatory T cell response

The paper identifies a widespread memory/effector T cell response and regulatory T cell presence against SpCas9 in healthy humans, with implications for gene therapy.

Distinct housing conditions reveal a major impact of adaptive immunity on the course of obesity-induced type 2 diabetes

This study shows that housing conditions affect adaptive immunity and metabolic outcomes in obesity-induced type 2 diabetes, with antigen-exposed mice showing improved glucose tolerance but liver inflammation.