Molecular BiologyDNA ResearchPeer-reviewed
3’ Branch Ligation: A Novel Method to Ligate Non-Complementary DNA to Recessed or Internal 3’OH Ends in DNA or RNA
Lin Wang, Yang Xi, Wenwei Zhang…·Yuan Jiang
The paper introduces a novel ligation method called 3BL that allows non-complementary DNA to be ligated to 3' ends of DNA or RNA, improving NGS library construction by reducing adapter dimer issues and increasing yield.