In vitro and In vivo Genetic Disease Modelling via NHEJ precise deletions using CRISPR/Cas9
S. Lopez-Manzaneda, I. Ojeda-Perez, N. Zabaleta, A. Garcia-Torralba, O. Alberquilla, R. Torres, R. Sanchez-Dominguez, L. Torella, E. Olivier, J. Mountford, J. C. Ramirez, J. A. Bueren, G. Gonzalez-Aseguinolaza, J. C. Segovia
The paper demonstrates that using two guideRNAs at optimal distance and orientation with CRISPR/Cas9 enables precise deletions for creating genetically reproducible disease models in human and mouse cells.
bioRxiv · Mar 25, 2020Bioengineering