BioengineeringbioRxiv
In vitro and In vivo Genetic Disease Modelling via NHEJ precise deletions using CRISPR/Cas9
S. Lopez-Manzaneda, I. Ojeda-Perez, N. Zabaleta…·J. C. Segovia
The paper demonstrates that using two guideRNAs at optimal distance and orientation with CRISPR/Cas9 enables precise deletions for creating genetically reproducible disease models in human and mouse cells.