Cryopreservation of the collector urchin embryo, Tripneustes gratilla
C. E. Westbrook, J. Daly, B. Bowen, M. Hagedorn
This study develops a cryopreservation protocol for T. gratilla embryos using 1M Me2SO and a cooling rate of 9.7°C/min, achieving 85.5% survival and 50.8% maturation to the 4-arm echinopluteus stage.
bioRxiv · Jan 22, 2024Zoology