J. N. Milstein

This paper improves axial optical tweezers by extending force range and reducing laser power through aberration correction and nonlinear response accounting.

This paper extends the Moran model to include spatial exclusion in microchannels, showing it alters fixation probabilities and fixation times compared to classical models.

The paper develops a machine learning method to quantify macromolecular complexes in SMLM data without requiring calibration.

This study defines and quantifies the dynamic range of single-molecule localization microscopy by analyzing photophysical and experimental factors affecting molecule counting accuracy.

This paper introduces an improved single-molecule assay for studying protein-DNA interactions, demonstrating enhanced stability and compatibility with Mg2+.

The paper introduces a pinhole optical trap that extends photobleaching lifetime by separating trap light from fluorescence illumination using wavefront engineering.

This paper introduces a method for molecular counting using single-molecule localization microscopy (SMLM) and benchmarks it on DNA origami grids to demonstrate its accuracy for optical -omics analysis.

This paper describes the calibration of a donut beam optical tweezers for single-molecule force spectroscopy, addressing polarization effects and trap height precision.

The paper develops a Bayesian method for molecular counting using localization microscopy, showing improved accuracy with multiple fluorophore labeling and applying it to quantify plasmid copy number variability in bacteria.