Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues
J. Stein, M. Ericsson, M. Nofal, L. Magni, S. Aufmkolk, R. B. McMillan, L. Breimann, C. P. Herlihy, S. D. Lee, A. Willemin, J. Wohlmann, L. Arguedas-Jimenez, P. Yin, A. Pombo, G. M. Church, C.-t. Wu
The paper introduces tkPAINT, a method combining TIRF microscopy and Tokuyasu cryosectioning to enhance DNA-PAINT for single-protein imaging and molecular counting with 3 nm precision across cells and tissues.
bioRxiv · Feb 5, 2024Cell Biology