J. Stein

A Bayesian Solution to Count the Number of Molecules within a Diffraction Limited Spot

This paper introduces a Bayesian method to estimate the number of fluorescent emitters in diffraction-limited spots, showing improved accuracy over existing methods.

Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues

The paper introduces tkPAINT, a method combining TIRF microscopy and Tokuyasu cryosectioning to enhance DNA-PAINT for single-protein imaging and molecular counting with 3 nm precision across cells and tissues.

DNA-PAINT single-particle tracking (DNA-PAINT-SPT) enables extended single-molecule studies of membrane protein interactions

This paper introduces dual-colour DNA-PAINT-SPT for live cell imaging, enabling tracking of membrane protein interactions and dimerisation with improved performance over single-dye SPT.

Calibration free counting of low molecular copy numbers in single DNA-PAINT localization clusters

This paper introduces lbFCS+, a method to absolutely count molecular copy numbers in DNA-PAINT localization clusters without calibration.

Non-equilibrium conditions inside rock pores drive fission, maintenance and selection of coacervate protocells

This study shows gas bubbles in heated rock pores drive the growth, fusion, division, and selection of coacervate microdroplets, simulating early evolutionary processes of protocells.

TCAIM controls effector T cell generation by preventing Mitochondria-Endoplasmic Reticulum Contact Site-initiated Cholesterol Biosynthesis

This study shows TCAIM prevents effector T cell differentiation by inhibiting mitochondria-ER contact sites that drive cholesterol biosynthesis and HIF1 activation.

Tracking Single Particles for Hours via Continuous DNA-mediated Fluorophore Exchange

This paper introduces a method to track single particles for hours using continuous DNA-mediated fluorophore exchange, overcoming photobleaching limitations.

Salivary gland macrophages assist tissue-resident CD8+ T cell immune surveillance

The paper uses imaging to show salivary gland TRM use unique motility and tissue macrophages assist in their surveillance.

In vivo function of the lipid raft protein Flotillin 1 during CD8+ T cell-mediated host surveillance

This study investigates the role of Flotillin 1 in CD8+ T cell migration and immune responses, finding minimal impact on cell behavior under physiological conditions.