K. K. Brower

This study addresses RT-PCR inhibition in picoliter double emulsion droplets by identifying lysate-induced probe cleavage as the main issue and showing heat lysis can alleviate it, enabling single-cell PCR in 20 pL droplets.

This paper systematically characterizes double emulsion droplets to predict their size and stability based on flow rates and buffer properties, enabling better design of bioassays.

The paper introduces Dropception, a method for high-throughput single-cell encapsulation and phenotyping using double emulsion picoreactors and FACS.

This paper introduces sdDE-FACS, a method for high-throughput phenotyping and sorting of double emulsion droplets using flow cytometry, achieving >70% single droplet recovery and efficient nucleic acid extraction.