Direct Nanopore Sequencing of Individual Full Length tRNA Strands
N. Thomas, V. Poodari, M. Jain, H. Olsen, M. Akeson, R. Abu-Shumays
This paper describes a method for direct tRNA sequencing using Oxford Nanopore MinION, achieving 76-92% full-length reads and detecting all 42 tRNA isoacceptors in E. coli.
bioRxiv · Apr 26, 2021Bioengineering