S. Covarrubias

The paper uses CRISPRi screens to identify the lncRNA LOUP as a regulator of macrophage differentiation and inflammatory signaling through epigenetic and peptide-mediated mechanisms.

The paper identifies inflammation-induced alternative first exon usage in immune genes, revealing a novel iron-regulated Aim2 isoform with a 5′UTR iron-responsive element.

The paper generates a HEK-293T cell line expressing murine GM-CSF and shows it supports dendritic cell and alveolar macrophage differentiation as an alternative to commercial GM-CSF.

The paper uses CRISPR screening to identify genes essential for macrophage viability and regulators of inflammatory pathways like NF-κB and TNF signaling.