Efficient Development of Platform Cell Lines Using CRISPR-Cas9 and Transcriptomics Analysis
A. T.-J. Kwon, K. Mohri, S. Takizawa, T. Arakawa, M. Takahashi, B. Kaczkowski, M. Furuno, H. Suzuki, S. Tagami, H. Mukai, E. Arner
The paper develops CRISPR-Cas9 mutants of U87MG cells to create platform cell lines for drug testing, finding that transcriptomic analysis helps identify clones with minimal phenotypic divergence from wild type.
bioRxiv · Sep 16, 2020 · PreprintBioengineering