Author

S. Tagami

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:protein folding & dynamics· 3 Assay:protein structure· 3 Research Focus:cell type annotation· 2 Organism:bacteria (other)· 1 Assay:bulk RNA-seq· 1 Research Focus:chromatin & epigenetic regulation· 1 Approach:deep learning· 1

Frequent Co-Authors

K. Y. J. Zhang· 2 shared S. Yagi· 2 shared A. Alfi· 1 shared A. K. Padhi· 1 shared A. Kumar· 1 shared A. Popov· 1 shared A. T.-J. Kwon· 1 shared B. Kaczkowski· 1 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 15 mutual co-author links

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2 shared papersBiochemistry

K. Y. J. Zhang

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Author profile →

5 papers

Recent·Most cited

Missing-link fold reveals the evolutionary pathway between RNA polymerase and ribosomal proteins

S. Yagi, S. Tagami

The paper identifies a missing-link fold, DZBB, that connects RNA polymerase and ribosomal proteins, showing rapid diversification from a common ancestor.

bioRxiv · Jul 5, 2023 · PreprintBiochemistry

Cell-free mutant analysis combined with structure prediction of a lasso peptide biosynthetic protein B2

A. Alfi, A. Popov, A. Kumar, K. Y. J. Zhang, S. Dubiley, K. Severinov, S. Tagami

The paper combines cell-free mutagenesis with AlphaFold2 structure prediction to validate the function of TfuB2, revealing a hydrophobic binding site for TfuB1.

bioRxiv · Apr 6, 2022Biochemistry

Hydrophobic-cationic peptides enhance RNA polymerase ribozyme activity by accretion

P. Li, P. Holliger, S. Tagami

This paper shows hydrophobic-cationic peptides enhance RNA polymerase ribozyme activity by concentrating RNA, with effects dependent on magnesium ion concentration.

bioRxiv · Feb 22, 2021Biochemistry

Seven amino acid types suffice to reconstruct the core fold of RNA polymerase

S. Yagi, A. K. Padhi, J. Vucinic, S. Barbe, T. Schiex, R. Nakagawa, D. Simoncini, K. Y. J. Zhang, S. Tagami

The paper reconstructs the DPBB fold using only seven amino acid types, suggesting it could have emerged with the early genetic code.

bioRxiv · Feb 22, 2021Biochemistry

Efficient Development of Platform Cell Lines Using CRISPR-Cas9 and Transcriptomics Analysis

A. T.-J. Kwon, K. Mohri, S. Takizawa, T. Arakawa, M. Takahashi, B. Kaczkowski, M. Furuno, H. Suzuki, S. Tagami, H. Mukai, E. Arner

The paper develops CRISPR-Cas9 mutants of U87MG cells to create platform cell lines for drug testing, finding that transcriptomic analysis helps identify clones with minimal phenotypic divergence from wild type.

bioRxiv · Sep 16, 2020 · PreprintBioengineering