Engineering of methionine-auxotroph Escherichia coli via parallel evolution of two enzymes from Corynebacterium glutamicum's direct-sulfurylation pathway enables its recovery in minimal medium
M. Gabay, I. Stern, N. Gruzdev, A. Cohen, L. A. Lifshits, T. Ansbacher, I. Yadid, M. Gal
This paper engineers methionine-auxotroph E. coli by evolving enzymes from C. glutamicum to enable growth in minimal medium.
Metabolic Engineering Communications · Jun 1, 2024Peer-reviewedBiochemistry