Engineering of methionine-auxotroph Escherichia coli via parallel evolution of two enzymes from Corynebacterium glutamicum's direct-sulfurylation pathway enables its recovery in minimal medium
M. Gabay, I. Stern, N. Gruzdev…·M. Gal
This paper engineers methionine-auxotroph E. coli by evolving enzymes from C. glutamicum to enable growth in minimal medium.