M. Harding

Development of a qPCR assay and a LAMP assay for Verticillium longisporum detection and a triplex qPCR assay for simultaneous detection of V. longisporum, Leptosphaeria biglobosa and L. maculans from canola samples

Detection and differentiation of Xanthomonas translucens pathovars translucens and undulosa from wheat and barley by duplex quantitative PCR

This paper develops two duplex qPCR systems to specifically detect and differentiate Xanthomonas translucens pathovars translucens and undulosa in wheat and barley.

Detection of Xanthomonas translucens pv. undulosa from wheat by quantitative PCR

This paper develops a qPCR protocol for detecting Xanthomonas translucens pv. undulosa in wheat, showing high specificity and sensitivity for bacterial detection.

Plasmodiophora brassicae infection threshold - How many resting spores are required for infection of canola (Brassica napus)

This study determines the minimum number of Plasmodiophora brassicae resting spores required to infect canola, finding thresholds as low as 100 spores, lower than previously reported.

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV)

The paper develops and evaluates a LAMP assay for detecting ToBRFV, finding it efficient, rapid, and more sensitive than rt-PCR.

Plasmodiophora brassicae in its environment-effects of temperature and light on resting spore survival in soil

This study investigates how temperature and light affect the survival of Plasmodiophora brassicae resting spores in soil, finding that lower temperatures and burial improve survival while UV light reduces viability.