M. Hiroshima

Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopy

This study uses super-resolution microscopy to show that EGFR and PI(4,5)P2 nanodomains coaggregate before EGF stimulation, and their interaction is regulated by phospholipase Cγ and PI(4,5)P2 levels.

Single-molecule tracking-based drug screening

This study uses single-molecule tracking to screen FDA-approved drugs for effects on EGFR mobility and clustering, identifying both known and novel compounds that impact EGFR function.

Membrane cholesterol interferes with tyrosine phosphorylation but facilitates the clustering and signal transduction of EGFR

This study shows that cholesterol affects EGFR mobility, clustering, and signaling by altering membrane subdomain structure and lipid composition.

Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors

The paper uses single-molecule imaging to assess ligand effects on GPCRs by monitoring diffusion dynamics on the cell surface, showing that diffusion coefficients correlate with receptor activity.