Y. Sako

Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopy

This study uses super-resolution microscopy to show that EGFR and PI(4,5)P2 nanodomains coaggregate before EGF stimulation, and their interaction is regulated by phospholipase Cγ and PI(4,5)P2 levels.

A SLiM-dependent conformational change in baculovirus IE1 controls its focus formation ability

This paper identifies a SLiM-dependent conformational change in baculovirus IE1 that controls its focus formation ability during different stages of infection.

Isolation and genomic and physiological characterization of Parageobacillus sp. G301, the isolate capable of both hydrogenogenic and aerobic carbon monoxide oxidation

This paper isolates and characterizes Parageobacillus sp. G301, a novel CO oxidizer capable of both hydrogenogenic and aerobic CO oxidation using both Ni-CODH and Mo-CODH.

Two closed conformations of CRAF require the 14-3-3 binding motifs and cysteine-rich domain to be intact in live cells

The paper uses smFRET to study CRAF conformational changes in live cells, identifying two closed conformations regulated by 14-3-3 binding and the cysteine-rich domain.

Fractal scaling of C. elegans behavior is shaped by insulin signaling

This study shows insulin signaling in C. elegans regulates fractal scaling of behavior, with glucose and genetic mutants affecting the power-law to exponential transition in residence-time distribution.

Membrane cholesterol interferes with tyrosine phosphorylation but facilitates the clustering and signal transduction of EGFR

This study shows that cholesterol affects EGFR mobility, clustering, and signaling by altering membrane subdomain structure and lipid composition.

Threonine phosphorylation regulates the molecular assembly and signaling of EGFR in cooperation with membrane lipids

This study shows that threonine phosphorylation in EGFR's JM domain switches its function from kinase activation dimers to scaffolding oligomers, influenced by membrane lipids.

Isolation and characterization of novel temperate virus Aeropyrum globular virus 1 infecting hyperthermophilic archaeon Aeropyrum

This paper isolates and characterizes a novel archaeal virus AGV1, showing it lacks integration into the host genome and replicates only under stress conditions.

Total workflows of the single-molecule imaging analysis in living cells: a tutorial guidance to the measurement of the drug effects on a GPCR

This paper provides a tutorial on using single-molecule imaging to analyze drug effects on GPCRs, including workflows and an open-source software tool.

C. elegans episodic swimming is driven by multifractal kinetics

This study identifies multifractal kinetics in C. elegans episodic swimming and links it to PKG regulation of behavioral transitions.

Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors

The paper uses single-molecule imaging to assess ligand effects on GPCRs by monitoring diffusion dynamics on the cell surface, showing that diffusion coefficients correlate with receptor activity.

Lipid-protein interplay in dimerization of the juxtamembrane domains of epidermal growth factor receptor

The paper investigates how lipid-protein interactions and Thr654 phosphorylation regulate EGFR JM dimerization and kinase activity.