T. Kobayashi

Structural and Dynamic Insights into the Biased Signaling Mechanism of the Human Kappa Opioid Receptor

This study investigates the structural dynamics of the human KOR-Gi signaling complex with G-protein-biased and balanced agonists, identifying key residues involved in signaling bias.

Flow zoometry of Drosophila

This paper introduces flow zoometry, a high-throughput screening method for Drosophila, enabling rapid, automated, and high-resolution analysis of whole animals.

A Novel Liver Cancer-Selective Histone Deacetylase Inhibitor Is Effective Against Hepatocellular Carcinoma and Induces Durable Responses with Immunotherapy

This paper identifies a novel HDAC inhibitor, STR-V-53, that effectively targets HCC and enhances immunotherapy responses in mouse models.

Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopy

This study uses super-resolution microscopy to show that EGFR and PI(4,5)P2 nanodomains coaggregate before EGF stimulation, and their interaction is regulated by phospholipase Cγ and PI(4,5)P2 levels.

A non-toxic equinatoxin-II reveals the dynamics of sphingomyelin in the cytosolic leaflet of the plasma membrane

The paper uses a non-toxic SM probe to show dynamic SM and cholesterol domains in the cytosolic leaflet of plasma membranes.

Combined blockade of CXCR4 and PD-1 enhances intratumoral dendritic cell activation and immune responses against HCC

This study shows that blocking CXCR4 and PD-1 together improves dendritic cell activation and immune responses against HCC in mouse models.

Liver fibrosis uncouples tumor control from survival after multikinase inhibitor immunotherapy in hepatocellular carcinoma

Liver fibrosis limits the survival benefit of multikinase inhibitor immunotherapy in hepatocellular carcinoma by promoting innate immune-mediated hepatotoxicity despite preserved tumor control.

A Th17 cell-intrinsic glutathione/mitochondrial-IL-22 axis protects against intestinal inflammation

This study identifies a Th17 cell-intrinsic glutathione/mitochondrial-IL-22 axis that protects against intestinal inflammation by maintaining barrier integrity and preventing mortality from C. rodentium infection.

Identification of a novel enhancer essential for Satb1 expression in TH2 cells and activated ILC2s.

The paper identifies a cis-regulatory enhancer critical for Satb1 expression in TH2 cells and activated ILC2s, revealing its role in type 2 immune responses.

Limited roles of Piezo mechanosensing channels in articular cartilage development and osteoarthritis progression

This study investigates the role of Piezo1 and Piezo2 in osteoarthritis progression by ablating these channels in mice, finding limited effects on joint development and OA severity.

Increased mitochondrial Ca2+ contributes to health decline with age and Duchene muscular dystrophy in C. elegans.

This study shows that reducing mitochondrial calcium influx in C. elegans improves age-related and muscular dystrophy muscle decline by mitigating mitochondrial dysfunction.

Reconstituted depolarization-induced Ca2+ release platform for skeletal muscle disease mutation validation and drug discovery

The paper establishes a reconstituted DICR platform in HEK293 cells to validate skeletal muscle disease mutations and test drug candidates.

Characterization of a KDM5 Small Molecule Inhibitor with Antiviral Activity against Hepatitis B Virus

This study identifies a KDM5 inhibitor with anti-HBV activity in vitro but not in vivo, highlighting challenges in translating epigenetic therapies to viral infections.

Lymphoma cells are circulating in blood of Enzootic Bovine Leukosis and clonality value of virus-infected cells is a useful information for the diagnostic test

The paper demonstrates that clonality values of BLV-infected cells in blood can reliably detect Enzootic Bovine Leukosis and are useful for diagnostic testing.

KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cells

This study shows that KIF24 depletion suppresses multipolar spindle formation and improves mitotic progression in PDAC cells with supernumerary centrosomes, independent of primary cilia.

Atypical small GTPase RABL3 interacts with RAB11 to regulate early ciliogenesis in human cells

This study identifies RABL3 as a regulator of early ciliogenesis by interacting with RAB11 to facilitate ciliary vesicle formation in human cells.

A Random Forest Classifier Uses Antibody Responses to Plasmodium Antigens to Reveal Candidate Biomarkers of the Intensity and Timing of Past Exposure to Plasmodium falciparum

The paper uses random forest models trained on antibody responses to Plasmodium antigens to classify adult samples by historical malaria transmission regions with high accuracy.

MOSPD2 a new endoplasmic reticulum-lipid droplet tether functioning in LD homeostasis

This study identifies MOSPD2 as an ER-lipid droplet tether that regulates LD homeostasis through its CRAL-TRIO domain.

Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP

This study elucidates the mechanism of intramembrane proteolysis by E. coli RseP through crystal structures and mutational analysis, revealing a gating mechanism for substrate entry.

Microplastic pollution of commercial fishes from coastal and offshore waters, Japan

The paper examines microplastic contamination in commercial fish from coastal and offshore waters near Kyushu, Japan, finding higher prevalence and larger sizes in pelagic species compared to demersal ones.

Membrane cholesterol interferes with tyrosine phosphorylation but facilitates the clustering and signal transduction of EGFR

This study shows that cholesterol affects EGFR mobility, clustering, and signaling by altering membrane subdomain structure and lipid composition.

Hypo-osmotic Stress Induces ATP Release via Volume-regulated Anion Channels in Undifferentiated Mammary Cells

The paper investigates ATP release patterns under hypotonic stress in mammary cells, identifying VRACs as a conduit for ATP release in undifferentiated cells.

Creation of X-linked Alport Syndrome Rat Model with Col4a5 Deficiency

This study creates a novel Alport syndrome rat model with Col4a5 deficiency to better understand renal disease mechanisms and therapeutic targets.

Spatiotemporal variations of surface water microplastics near Kyushu, Japan: A quali-quantitative analysis

This study quantifies and analyzes spatiotemporal variations in microplastic abundance, size, color, and polymer type near Kyushu, Japan, revealing significant seasonal and spatial differences.

Molecular basis for gating of cardiac ryanodine receptor: underlying mechanisms for gain- and loss-of function mutations

This study identifies two gating mechanisms of RyR2 and shows how mutations affect channel function.

Zinc-binding motif acts as an oxidative stress sensor to regulate TRPM7 channel activity

This study identifies the zinc-binding motif in TRPM7 as critical for oxidative stress sensing and Mg2+-dependent regulation of channel activity.

A novel RyR1 inhibitor prevents and rescues sudden death in a mouse model of malignant hyperthermia and heat stroke

This study introduces a novel RyR1 inhibitor that prevents and rescues MH and heat stroke in mouse models, offering improved solubility and clearance compared to dantrolene.

Temperature-dependent fasciation mutants connect mitochondrial RNA processing to the control of cell proliferation during lateral root morphogenesis

Generation of human bronchial organoids for SARS-CoV-2 research

This paper generates human bronchial organoids that support SARS-CoV-2 infection and drug testing, showing reduced viral load with TMPRSS2 inhibition.

Genetic diversity of Anopheles coustani in high malaria transmission foci in southern and central Africa

This study examines genetic variation and malaria transmission potential of Anopheles coustani in high transmission areas of Zambia and the DRC.