Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
M. Matsuo-Takasaki, S. Kambayashi, Y. Hemmi, T. Wakabayashi, T. Shimizu, Y. An, H. Ito, K. Takauchi, M. Ibuki, T. Kawashima, R. Masayasu, M. Suzuki, N. Nishishita, Y. Kawai, M. Umekage, , Y. Hayashi
This study develops a suspension culture method for hiPSCs using PKCβ and Wnt inhibitors to prevent spontaneous differentiation, enabling scalable and stable clinical-grade hiPSC production.
bioRxiv · Oct 8, 2023Bioengineering