Y. Hayashi

Label-free imaging of intracellular structures in living mammalian cells via external apodization phase-contrast microscopy

This study introduces ExAPC microscopy for label-free visualization of intracellular structures, showing its ability to resolve biomolecular condensates, lipid droplets, and mitochondria with high spatiotemporal resolution.

Beyond Basic Characterization and Omics: Immunomodulatory Roles of Platelet-Derived Extracellular Vesicles Unveiled by Functional Testing

This study investigates the immunomodulatory roles of platelet-derived extracellular vesicles by comparing their functional effects and omics profiles across different activation methods.

A liquid-like coat mediates chromosome clustering during mitotic exit

This study shows Ki-67 switches from repelling to attracting chromosomes during mitotic exit, forming a liquid-like coat that drives chromosome clustering through phase separation.

Integration of shoot-derived polypeptide signals by root TGA transcription factors is essential for survival under fluctuating nitrogen environments

This study shows that TGA1 and TGA4 transcription factors integrate shoot-derived polypeptide signals to regulate nitrate uptake in response to fluctuating nitrogen environments.

Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

This study develops a suspension culture method for hiPSCs using PKCβ and Wnt inhibitors to prevent spontaneous differentiation, enabling scalable and stable clinical-grade hiPSC production.

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

This paper introduces genetically encoded multimeric tags for localizing intracellular proteins in cryo-EM, demonstrating their use in human cells with drug-controlled labeling.

Image-based quantification of Arabidopsis thaliana stomatal aperture from leaf images

This paper presents an automated image analysis pipeline for quantifying stomatal aperture in Arabidopsis thaliana leaves using brightfield microscopy images.

Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes

This study uses patient-derived iPSCs to identify retinoids as potential therapeutics for Wilson's disease by rescuing ceruloplasmin secretion and reducing oxidative stress.

Structure and Function of Gli123 Involved in Mycoplasma mobile Gliding

This study investigates the structure and function of Gli123, a surface protein essential for Mycoplasma mobile gliding, revealing its conformational changes and evolutionary implications.

Gene expression profiles of chemosensory genes of termite soldier and worker antennae

The paper compares gene expression in worker and soldier antennae of termites to identify chemosensory genes involved in caste-specific social behaviors.

Thoracic crop formation is spatiotemporally coordinated with flight muscle histolysis during claustral colony foundation of a Lasius japonicus queen

Hematopoietic stem and progenitor cells integrate Bacteroides-derived innate immune signals to promote gut tissue repair

This study shows that Bacteroides activates HSPCs in the BM during acute gut inflammation to promote tissue repair, but impairs HSC function in chronic inflammation and aging.

Aberrant accumulation of NIK promotes tumorigenicity by dysregulating post-translational modifications in breast cancer

This study identifies NIK upregulation in breast cancer due to translational dysregulation, which promotes tumorigenicity by activating the non-canonical NF-κB pathway.

Mechanosensory trichome cells evoke a mechanical stimuli-induced immune response in plants

The paper shows that trichome cells in Arabidopsis detect mechanical stimuli from raindrops, triggering calcium waves that activate immune responses against pathogens.

Evolutionary transition of doublesex regulation in termites and cockroaches: from sex-specific splicing to male-specific transcription

This study investigates the evolutionary transition of dsx regulation in termites and cockroaches, finding a shift from sex-specific splicing to male-specific transcription in termites.

Chromatin loading of MCM hexamers is associated with di-/tri-methylation of histone H4K20 toward S phase entry

This study investigates MCM hexamer states during the cell cycle in human cells and links their formation to histone H4K20 methylation levels, showing that di/trimethyl-H4K20 correlates with double MCM hexamer formation.

CaMKII binds both substrates and activators at the active site

This study challenges a longstanding model of CaMKII activation by showing that activators and substrates bind to a single site rather than distinct S- and T-sites.

Tracing the in Vivo Fate of Nanoparticles with a "Non-Self" Biological Identity

The paper investigates the in vivo fate of nanoparticles with a non-self protein corona in zebrafish embryos, showing rapid sequestration and inflammatory effects.

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

This study identifies soft corona proteins on nanoparticles and shows their role in modulating nanoparticle-cell association despite weak interactions.

Phosphorylation of Elovl5 changes its substrate preference to synthesize Mead acid in response to essential fatty acid deficiency

Molecular determinants of Arc oligomerization and formation of virus-like capsids

This paper identifies a 28-amino-acid region in Arc critical for oligomerization and virus-like capsid formation, showing that exogenous RNA enhances assembly.

Functionalized mesoporous silica nanoparticles for innovative boron-neutron capture therapy of resistant cancers

This study develops B-MSNs for BNCT to treat chondrosarcomas, showing DNA damage and cell death in radio-resistant CSCs.

Electrically induced bacterial membrane potential dynamics correspond to cellular proliferation capacity

This study shows that electrical stimulation of bacteria causes opposite membrane potential dynamics depending on their proliferative capacity, enabling single-cell detection of bacterial growth and antimicrobial sensitivity.

GUN1 regulates tetrapyrrole biosynthesis

This study identifies GUN1's role in chloroplast-to-nucleus retrograde signaling by showing it binds heme, affects tetrapyrrole biosynthesis, and enhances FC1 activity.

Structure of monomeric full-length ARC sheds light on molecular flexibility, protein interactions, and functional modalities

This study determines the structure of monomeric full-length ARC, revealing its compact conformation and interaction sites for postsynaptic proteins and phospholipid membranes.

Cas9/CRISPR genome editing to demonstrate the contribution of Cyp51A Gly138Ser to azole resistance in Aspergillus fumigatus

This study uses CRISPR/Cas9 to demonstrate that the Cyp51A Gly138Ser SNP increases azole resistance in Aspergillus fumigatus by reducing susceptibility.

Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a Light-Responsive Polymer

The paper develops a laser-based system for automated removal of differentiated cells from pluripotent stem cell cultures using light-responsive polymers and machine learning.