Y. An

Amidase and Lysozyme Dual Functions in TseP Reveal a New Family of Chimeric Effectors in the Type VI Secretion System

This paper identifies TseP as a dual amidase-lysozyme T6SS effector with potential for combating antimicrobial resistance.

Proteasome inhibition induces DNA methylation alteration by attenuating the synthesis of DNA methyltransferase 1 and 3B in colorectal cancer

This study shows proteasome inhibition alters DNA methylation in colorectal cancer by reducing DNMT1 and DNMT3B synthesis via AKT/mTOR inactivation.

Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival

This study transplants human iPSC-derived pancreatic progenitors into Pdx1-deficient mice, extending their lifespan and demonstrating functional engraftment of human pancreatic cells.

VPS41 deletion triggers progressive loss of insulin stores and downregulation of beta-cell identity

This study shows that VPS41 deletion causes insulin degradation and loss of beta-cell identity in mice, with lysosomal inhibition rescuing insulin content.

Efficient cloning of genes for wheat yield component traits from QTLs via sequencing of the RIL population

This study develops a method to clone genes for wheat yield traits by sequencing RIL populations, identifying and validating 11 candidate genes from 77 QTLs.

LLPS condensates of Fha initiate the inside-out assembly of the type VI secretion system

This study identifies Fha as the initiator of T6SS assembly in Acidovorax citrulli, showing it forms LLPS condensates that recruit T6SS components, revealing an inside-first assembly model.

A new method for the reproducible development of aptamers (Neomers)

This paper introduces a new aptamer selection method called Neomers, which improves reproducibility and specificity by mimicking immune tolerance through structured random nucleotide libraries.

Repression of mRNA translation initiation by GIGYF1 via blocking the eIF3-eIF4G1 interaction

This study identifies GIGYF1 as a repressor of mRNA translation by disrupting the eIF3-eIF4G1 interaction, enhancing host immune response by derepressing Ifnb1 mRNA.

Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

This study develops a suspension culture method for hiPSCs using PKCβ and Wnt inhibitors to prevent spontaneous differentiation, enabling scalable and stable clinical-grade hiPSC production.

SARS-CoV-2 Omicron BA.2.86: less neutralization evasion compared to XBB sub-variants

This study compares the immune evasion of SARS-CoV-2 Omicron BA.2.86 to XBB sub-variants, finding BA.2.86 shows less neutralization evasion.

Neutralization of SARS-CoV-2 EG.5/EG.5.1 by sera from ZF2001 RBD-dimer and its next-generation vaccines

This study evaluates the neutralization of SARS-CoV-2 EG.5/EG.5.1 by sera from vaccinated individuals and breakthrough infections, finding that next-generation vaccines offer better protection against these variants.

Active learning of the thermodynamics-dynamics tradeoff in protein condensates

This study uses simulations to show that condensate dynamics and thermodynamics are correlated, then identifies sequences that break this correlation to design tunable biomaterials.

A hierarchical strategy to decipher protein dynamics in vivo with chemical cross-linking mass spectrometry

This paper introduces a hierarchical strategy using CXMS and AlphaFold2 to analyze protein dynamics in vivo, focusing on IDPs and multi-domain proteins.

Pooled CRISPR screening of high-content cellular phenotypes by ghost cytometry

The paper introduces a ghost cytometry-based method for fast pooled CRISPR screening of high-content cellular phenotypes, including fluorescence and label-free modes, enabling genome-wide analysis of gene effects on cellular processes.

Integrated transcriptome and lineage analyses reveal novel catecholaminergic cardiomyocytes contributing to the cardiac conduction system in murine heart

This study identifies a novel catecholaminergic cardiomyocyte population expressing Dbh that contributes to the murine cardiac conduction system through transcriptomic, genetic, and optogenetic analyses.

Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes

This study uses patient-derived iPSCs to identify retinoids as potential therapeutics for Wilson's disease by rescuing ceruloplasmin secretion and reducing oxidative stress.

Genetic, Clinical Underpinnings of Brain Change Along Two Neuroanatomical Dimensions of Clinically-defined Alzheimer's Disease

This study identifies two brain atrophy dimensions in Alzheimer's disease using Surreal-GAN and links them to genetic and clinical factors, revealing distinct pathological mechanisms.

HDL-C as a potential medium between depletion of Lachnospiraceae genera and hypertension under high-calorie diet

This study links gut microbial depletion of Lachnospiraceae genera to lower HDL-C and hypertension in a high-calorie diet population, suggesting HDL-C as a mediator.

Global Spatial Transcriptome of Macaque Brain at Single-Cell Resolution

The paper creates a 3-D cell type atlas of the monkey brain using single-cell spatial transcriptomics and identifies molecular markers for neocortical layers and subregions.

Improving the Identification Coverage of Protein Interactome by Enhancing the Click Chemistry-based Cross-linking Enrichment Efficiency

This paper evaluates click chemistry-based methods for cross-linking peptide enrichment, finding that acid-cleavable protein-based conjugation identifies the most cross-linked peptides.

Spatially resolved profiling of protein conformation and interactions by biocompatible chemical cross-linking in living cells

This paper introduces SPACX, a method to profile protein conformation and interactions in living cells, revealing subcellular-specific heterogeneity and functional differences in PTEN.

Stable Flow-induced Expression of KLK10 Inhibits Endothelial Inflammation and Atherosclerosis

This study identifies KLK10 as a flow-sensitive endothelial protein that inhibits inflammation and atherosclerosis through a PAR1/2-dependent mechanism involving HTRA1 regulation.

Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells

This study uses single-cell RNA sequencing to identify five MSC subpopulations, revealing distinct developmental trajectories and functional roles in self-renewal and immunoregulation.

APOE ε4 allele advances the age-dependent decline of amyloid β clearance in the human cortex

This study shows that the APOE ε4 allele causes earlier age-related decline in amyloid β clearance in the human cortex, supporting early intervention strategies for Alzheimer's disease.

A tandem-repeat dimeric RBD protein-based COVID-19 vaccine ZF2001 protects mice and nonhuman primates

ZF2001, a tandem-repeat dimeric RBD protein vaccine, induces strong immune responses and protects mice and non-human primates from SARS-CoV-2 infection.

Microbial tryptophan catabolism affects the vector competence of Anopheles

This study shows that gut microbiota in Anopheles stephensi affects tryptophan metabolism, with Pseudomonas alcaligenes reducing 3-HK to protect against Plasmodium infection.

Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat RBD of SARS-CoV-2 spike protein protects mice against COVID-19

This study evaluates a recombinant chimpanzee adenovirus vaccine expressing a dimeric tandem-repeat RBD of SARS-CoV-2, showing enhanced immune responses and protection in mice.

Identification and Characterization of a Residual Host Cell Protein Hexosaminidase B Associated with N-glycan Degradation During the Stability Study of a Therapeutic Recombinant Monoclonal Antibody Product

The paper identifies Hexosaminidase B as a residual host cell protein causing N-glycan degradation in a monoclonal antibody under stability conditions and develops strategies to mitigate its impact.

CECR2 Drives Breast Cancer Metastasis by Suppressing Macrophage Inflammatory Responses

This study identifies CECR2 as a driver of breast cancer metastasis by suppressing macrophage inflammatory responses, offering a therapeutic target for metastatic disease.

Glucose-mediated expansion of a gut commensal bacterium promotes Plasmodium infection through alkalizing mosquito midgut

The paper shows dietary glucose promotes Plasmodium infection by expanding a gut bacterium that alkalizes mosquito midgut, enhancing parasite development.