BioengineeringbioRxiv
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
M. Matsuo-Takasaki, S. Kambayashi, Y. Hemmi…·Y. Hayashi
This study develops a suspension culture method for hiPSCs using PKCβ and Wnt inhibitors to prevent spontaneous differentiation, enabling scalable and stable clinical-grade hiPSC production.
DOI 10.7554/elife.89724.3