M. Tisma

DNA supercoiling enhances DNA condensation by ParB proteins

This study shows DNA supercoiling enhances ParB-DNA condensation, forming partition complexes at lower ParB concentrations than on relaxed DNA.

Topology-dependent DNA binding

This study presents a quantitative model showing how DNA topology modulates binding, with experiments using fluorescent intercalators and psoralen to demonstrate topology-dependent effects.

Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation

This study investigates how ParB-DNA interactions form a dynamic partition condensate for bacterial chromosome segregation using in vitro experiments and simulations.

Direct observation of a crescent-shape chromosome in Bacillus subtilis

The paper visualizes the crescent shape of the Bacillus subtilis chromosome in live cells and identifies key proteins involved in its structure.

ParS-independent recruitment of the bacterial chromosome-partitioning protein ParB

This study shows ParB can recruit itself to DNA independently of parS, enabling long-distance chromosome segregation in bacteria.

Direct visualization of four diffusive LexA states controlling SOS response strength during antibiotic treatment

This study visualizes four diffusive LexA states in E. coli during antibiotic treatment, revealing their dynamics and role in SOS response regulation.