Engineering a Single Extracellular Vesicle Protein and RNA Assay (siEVPRA) via In Situ Fluorescence Microscopy in a UV Micropatterned Array
J. Zhang, X. Wang, X. Y. Rima, L. T. H. Nguyen, K. Huntoon, Y. Ma, N. Walters, K. J. Kwak, M. J. Yoon, D. Lee, Y. Wang, J. Ha, K. Scherler, S. Fallen, I. Lee, , E. Reategui
The paper introduces siEVPRA, a method to detect proteins, mRNAs, and miRNAs in single EVs using fluorescence microscopy, showing high sensitivity and clinical utility in GBM samples.
bioRxiv · Aug 5, 2022Bioengineering