R. Kiewisz

This study uses electron tomography to show that MT minus ends in mitotic spindles are heterogeneous, with MCRS1 modulating their open/closed states.

This paper uses electron tomography to reveal the 3D structure of kinetochore-fibers in human mitotic spindles, showing nine KMTs per fiber and structural variations along their length.

This study investigates how kinetochore microtubules (KMTs) organize in human mitotic spindles using electron tomography, experiments, and modeling, finding that KMTs grow along defined trajectories and predominantly nucleate de novo at kinetochores.

The paper presents a semi-automatic workflow for aligning and stitching filamentous structures in serial-section electron tomography data to improve 3D reconstruction of microtubules in spindles.

The paper identifies mechanisms in C. elegans male meiosis that enable efficient segregation of paired and lagging chromosomes during anaphase.

The paper measures central spindle microtubule movement during chromosome segregation in human and C. elegans spindles, finding they slide apart at the same speed as chromosomes and are strongly coupled to them.