BiochemistrybioRxivPreprint
Precise DNA Cloning via PAMless CRISPR-SpRYgests
K. A. Christie, J. A. Guo, R. A. Silverstein…·B. P. Kleinstiver
This paper introduces SpRYgests, a CRISPR-based DNA cloning method that enables precise cleavage at any sequence, improving cloning workflows and reducing costs compared to restriction enzymes.
DOI 10.1101/2022.01.11.474553