Author

M. Mabuchi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Approach:genetic perturbation / CRISPR· 2 Research Focus:clustering· 1 Assay:CRISPR screen· 1 Assay:DNA methylation· 1 Research Focus:drug response / drug discovery· 1 Research Focus:gene regulatory network inference· 1 Organism:human· 1

Frequent Co-Authors

G. B. Robb· 4 shared J. L. Curcuru· 2 shared R. T. Fuchs· 2 shared A. Kadina· 1 shared A. Mendez-Mancilla· 1 shared B. P. Kleinstiver· 1 shared C. Alarcon· 1 shared C. Venclovas· 1 shared

Activity by Year

2018-2022 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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4 shared papersBiochemistry

G. B. Robb

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4 papers

Recent·Most cited

Precise DNA Cloning via PAMless CRISPR-SpRYgests

K. A. Christie, J. A. Guo, R. A. Silverstein, R. M. Doll, M. Mabuchi, H. E. Stutzman, L. Ma, G. B. Robb, B. P. Kleinstiver

This paper introduces SpRYgests, a CRISPR-based DNA cloning method that enables precise cleavage at any sequence, improving cloning workflows and reducing costs compared to restriction enzymes.

bioRxiv · Jan 11, 2022 · PreprintBiochemistry

Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

A. Mendez-Mancilla, H.-H. Wessels, M. Legut, A. Kadina, M. Mabuchi, J. Walker, G. B. Robb, K. Holden, N. E. Sanjana

The paper identifies chemically modified crRNAs that enhance CRISPR-Cas13 knockdown efficiency and half-life in human cells.

bioRxiv · May 12, 2021Bioengineering

Biochemically diverse CRISPR-Cas9 orthologs

G. Gasiunas, J. K. Young, T. Karvelis, D. Kazlauskas, T. Urbaitis, M. Jasnauskaite, M. Grusyte, S. Paulraj, P.-H. Wang, Z. Hou, S. K. Dooley, M. Cigan, C. Alarcon, N. D. Chilcoat, G. Bigelyte, , V. Siksnys

The paper characterizes the biochemical diversity of CRISPR-Cas9 orthologs, identifying varied PAM recognition and gRNA requirements across 79 Cas9 proteins.

bioRxiv · Apr 29, 2020 · PreprintBiochemistry

Staphylococcus aureus Cas9 is a multiple-turnover enzyme

P. Yourik, R. T. Fuchs, M. Mabuchi, J. L. Curcuru, G. B. Robb

This paper compares the catalytic activity of SpyCas9 and SauCas9, finding that SauCas9 is a multiple-turnover enzyme with distinct DNA cleavage properties.

bioRxiv · Jun 7, 2018Biochemistry