G. B. Robb

Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein

This paper describes an RNA capping enzyme-T7 RNA polymerase fusion protein that efficiently generates capped synthetic mRNA in a one-step process, achieving up to 95% Cap-1 incorporation.

Streamlined DNA template preparation and co-transcriptional 5' capped RNA synthesis enabled by solid-phase catalysis

This paper demonstrates a solid-phase enzymatic platform for streamlined mRNA synthesis, enabling efficient and continuous production of capped RNA without purification steps.

Biochemical characterization of mRNA capping enzyme from Faustovirus

This study characterizes a single-subunit RNA capping enzyme from Faustovirus, showing it efficiently generates Cap-0 structures and has advantageous properties for synthetic mRNA manufacturing.

RNase H-based analysis of synthetic mRNA 5-prime cap incorporation

The paper describes methods to analyze synthetic mRNA 5' cap incorporation using RNase H-based cleavage and simplified purification techniques.

Precise DNA Cloning via PAMless CRISPR-SpRYgests

This paper introduces SpRYgests, a CRISPR-based DNA cloning method that enables precise cleavage at any sequence, improving cloning workflows and reducing costs compared to restriction enzymes.

Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

The paper identifies chemically modified crRNAs that enhance CRISPR-Cas13 knockdown efficiency and half-life in human cells.

Biochemically diverse CRISPR-Cas9 orthologs

The paper characterizes the biochemical diversity of CRISPR-Cas9 orthologs, identifying varied PAM recognition and gRNA requirements across 79 Cas9 proteins.

Staphylococcus aureus Cas9 is a multiple-turnover enzyme

This paper compares the catalytic activity of SpyCas9 and SauCas9, finding that SauCas9 is a multiple-turnover enzyme with distinct DNA cleavage properties.