R. Seidel

Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB

This study investigates how HROB regulates the DNA unwinding activity of the MCM8-9 helicase complex through structural and biochemical analyses.

The energy landscape for R-loop formation by the CRISPR-Cas Cascade complex

This paper uses ultrafast DNA unwinding experiments to study the energy landscape of R-loop formation by the Cascade complex, revealing a downhill bias followed by an uphill bias and showing base flips and mismatches modulate the landscape.

Dynamic interplay between target search and recognition for the Cascade surveillance complex of type I-E CRISPR-Cas systems

This study investigates how the Type I-E CRISPR-Cas Cascade complex searches and recognizes DNA targets, showing that recognition probabilities are low and increased by DNA supercoiling and limited 1D diffusion.

A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex

The paper develops a quantitative model to predict CRISPR-Cas Cascade off-target recognition dynamics using single-molecule experiments and shows it accurately captures target recognition with mutations.

Probing the stability of the SpCas9-DNA complex after cleavage

This study investigates the stability of SpCas9-DNA complexes after cleavage using single-molecule magnetic tweezers, finding that post-cleavage states are more stable than pre-cleavage states.

CtIP promotes the motor activity of DNA2 to accelerate long-range DNA end resection

This study shows phosphorylated CtIP enhances DNA2's motor activity, promoting RPA-coated ssDNA degradation and linking short and long-range DNA resection.

Competing interactions modulate the activity of Sgs1 during DNA end resection

This study investigates how Sgs1's activity is modulated by competing interactions with Dna2, RPA, and Top3-Rmi1 during DNA end resection in yeast.