Autoinhibited kinesin-1 adopts a hierarchical folding pattern
Z. Tan, Y. Yue, F. da Veiga Leprevost…·M. A. Cianfrocco
This study determines the structure of autoinhibited kinesin-1 using XL-MS, EM, and AlphaFold, showing how light chains stabilize the inhibited state and how activation requires disrupting multiple domain interactions.