Development of a Method Combining Peptidiscs and Proteomics to Identify, Stabilize and Purify a Detergent-Sensitive Membrane Protein Assembly
J. W. Young, I. S. Wason, Z. Zhao, S. Kim, H. Aoki, S. Phanse, D. G. Rattray, L. J. Foster, M. Babu, F. Duong
The paper develops a method using peptidiscs and proteomics to stabilize and identify detergent-sensitive membrane protein complexes, demonstrating its effectiveness with the E. coli Sec system.
bioRxiv · Mar 3, 2022 · PreprintBiochemistry