Snorkel-tag Based Affinity Chromatography for Recombinant Extracellular Vesicle Purification
M. R. Bobbili, A. Gorgens, Y. Yan, S. Vogt, D. Gupta, G. Corso, S. Barbaria, C. Patrioli, S. Weilner, M. Pultar, J. Jacak, M. Hackl, M. Schosserer, R. Grillari, J. Kjems, , J. Grillari
This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.
bioRxiv · Apr 12, 2024Bioengineering