D. Gupta

Clonal spheroids capture functional and genetic heterogeneity of head and neck cancer

This study generates and characterizes single-cell-derived spheroids from head and neck cancer cells to reveal functional and genetic heterogeneity.

Snorkel-tag Based Affinity Chromatography for Recombinant Extracellular Vesicle Purification

This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.

Next-Gen Profiling of Tumor-resident Stem Cells using Machine learning

This paper introduces OSCORP, a machine learning method that identifies cancer stem cells with 99% accuracy using bulk RNAseq data, revealing CSC states and their clinical and transcriptomic signatures across 22 cancer types.

Nested biofabrication: Matryoshka-inspired Intra-embedded Bioprinting

This paper introduces Intra-Embedded Bioprinting (IEB), a method for creating complex, nested organ models with internal anatomical features using a xanthan gum-based material.

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

This paper introduces a versatile, photocrosslinkable silicone composite for 3D printing, demonstrating its use as a support bath and extrudable ink for creating complex microfluidic devices and biomedical constructs.

Multimodal engineering of extracellular vesicles for efficient intracellular protein delivery

This paper develops VEDIC and VFIC systems for efficient intracellular protein delivery using engineered extracellular vesicles, achieving high recombination and genome editing efficiency in vitro and in vivo.

MmcA is an electron conduit that facilitates both intracellular and extracellular electron transport in Methanosarcina acetivorans

This study identifies MmcA as an electron conduit in Methanosarcina acetivorans that supports both methanogenesis and dissimilatory metal reduction.

Novel endogenous engineering platform for robust loading and delivery of functional mRNA by extracellular vesicles

This paper develops an mRNA delivery system using extracellular vesicles engineered with RNA-binding domains and fusogenic moieties, showing improved efficacy in a melanoma model.

Trial-history biases in evidence accumulation can give rise to apparent lapses

The paper shows that trial-history biases and apparent lapses in decision-making can both result from a single cognitive process involving misbeliefs about non-stationarity.

High-throughput microgel biofabrication via air-assisted co-axial jetting for cell encapsulation, 3D bioprinting, and scaffolding applications

This paper develops a high-throughput method for producing microgels with tunable properties and cell encapsulation capabilities for bioprinting and tissue engineering applications.

Characterization of Plasmodium falciparum RAP domain proteins-RAP291 and RAP070, and their association with ribosomal RNAs

This study characterizes two Plasmodium falciparum RAP domain proteins, showing they bind ribosomal RNAs and associate with the MSP-1 complex, and inhibit merozoite invasion.

The dual role of a multi-heme cytochrome in methanogenesis: MmcA is important for energy conservation and carbon metabolism in Methanosarcina acetivorans

This study investigates the roles of the Rnf complex and MmcA in Methanosarcina acetivorans, showing MmcA is essential for acetoclastic methanogenesis but not for growth on methylated compounds, and reveals alternative ferredoxin regeneration pathways.

The non-catalytic role of DNA polymerase epsilon in replication initiation in human cells

This study investigates the non-catalytic role of POLE1 in DNA replication initiation in human cells, finding that its C-terminal domain can rescue replication initiation through interaction with POLE2.

SARS-CoV-2 Variant Delta Potently Suppresses Innate Immune Response and Evades Interferon-Activated Antiviral Responses

This study shows that the SARS-CoV-2 Delta variant suppresses innate immune responses and evades interferon activation more effectively than earlier variants.

An Archaea-Specific c-type Cytochrome Maturation Machinery is Crucial for Methanogenesis in Methanosarcina acetivorans

This study identifies an Archaea-specific c-type cytochrome maturation machinery essential for methanogenesis in Methanosarcina acetivorans, showing its conservation and substrate-specific roles.

Regulation of eIF2α Phosphorylation by MAPKs Influences Polysome Stability and Protein Translation

This study shows that p38 MAPK and ERK1/2 regulate polysome stability and translation through integrated stress response pathways, with dual inhibition causing significant polysome collapse.

Artemisinin acts by inhibiting Plasmodium falciparum Ddi1, a retropepsin, resulting into the accumulation of ubiquitinated proteins

This study shows that Artemisinin inhibits Plasmodium falciparum Ddi1, leading to ubiquitinated protein accumulation and impaired damage recovery.

SARS-CoV-2 Infection Causes Strong mTORC1 Inhibition and Massive Polysome Collapse

This study shows SARS-CoV-2 infection inhibits mTORC1 and causes polysome collapse, but not through eIF2 phosphorylation, and allows viral mRNA translation while suppressing host mRNA translation.

N440K variant of SARS-CoV-2 has Higher Infectious Fitness

The paper investigates the N440K variant of SARS-CoV-2, finding it produces significantly higher infectious titers than two other strains in cell cultures.

Inactivation of SARS-CoV-2 by β-propiolactone Causes Aggregation of Viral Particles and Loss of Antigenic Potential

This study examines the effects of β-propiolactone on SARS-CoV-2 inactivation, finding that higher concentrations cause protein loss, antigenic degradation, and virion aggregation.

Development of Equine Immunoglobulin Fragment F(ab')2 with High Neutralizing Capability against SARS-CoV-2

This study develops equine-derived F(ab')2 antibodies with high neutralizing capability against SARS-CoV-2, showing effectiveness against viral variants.

Decisive Role of Polymer-BSA Interactions in Biofilm Substrates on Philicity and EPS Composition

This study investigates how polymer-BSA interactions affect biofilm philicity and EPS composition by analyzing bacterial growth on different polymers with and without BSA conditioning.

Identification of COVID-19 prognostic markers and therapeutic targets through meta-analysis and validation of Omics data from nasopharyngeal samples

This study identifies prognostic markers and therapeutic targets for COVID-19 by analyzing Omics data from nasal and BALF samples, finding S100 family genes and TXN as key factors, and validating Auranofin as a potential treatment.

Generating controlled gusts using vortex rings

This paper introduces a vortex ring-based method to generate precise, controllable, and repeatable gusts for studying flight and swimming dynamics in animals and vehicles.

Multiparametric Profiling of Single Nanoscale Extracellular Vesicles by Combined Atomic Force and Fluorescence Microscopy: Correlation and Heterogeneity in Their Molecular and Biophysical Features

This study uses combined atomic force and fluorescence microscopy to analyze molecular and biophysical features of single extracellular vesicles from different cell lines, revealing correlations and heterogeneity in their properties.

Transcriptome analysis in the maize-Ustilago maydis interaction identifies maize-line-specific activity of fungal effectors

The paper identifies maize-line-specific virulence functions of Ustilago maydis effectors through RNA-Seq and CRISPR/Cas9 mutants, revealing auxin-related processes as a target.

Retinoic Acid Inducible Gene-I like Receptors Activate Snail and Slug to Limit RNA Viral Infections

The paper shows that RLRs activate Snail and Slug to limit RNA viral infections by regulating their transcription and enhancing the antiviral response.

Design and development of a vortex ring generator to study the impact of the ring as a gust

The paper develops a vortex ring generator to create controlled aerodynamic gusts and studies its impact on flying insects and other organisms.

Genetic redundancy in iron and manganese transport in the metabolically versatile bacterium Rhodopseudomonas palustris TIE-1

This study investigates Fe and Mn transport in Rhodopseudomonas palustris TIE-1, showing functional redundancy among transporters and their regulation by Fe and oxygen availability.

Plasmodium DEH is ER-localized and crucial for oocyst mitotic division during malaria transmission

This study identifies DEH as an ER-localized protein essential for oocyst mitotic division in Plasmodium berghei, disrupting sporogony and parasite transmission.