M. Hackl

Snorkel-tag Based Affinity Chromatography for Recombinant Extracellular Vesicle Purification

This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.

Characterizing the Cellulose Binding Interactions of Type-A Carbohydrate-Binding Modules using Acoustic Force Spectroscopy

This study uses acoustic force spectroscopy to measure rupture forces of CBM3a and CBM64 and their mutants, quantifying how mutations affect cellulose binding interactions.

Site-specific Effector Protein Functionalization to Create Bead-based Avidity Model Systems

This paper introduces a method to create bead-based avidity model systems using effector beads for measuring avidity effects in cell-cell interactions and drug potency assessment.

Multivariable Graphical User Interface for Simulation of Tethered Particle Motion

The paper develops a MATLAB simulation package for tethered particle motion (TPM) to model and visualize biophysical systems, validated against experimental techniques like Acoustic Force Spectroscopy.

Acoustic Force Spectroscopy Reveals Subtle Differences in Cellulose Unbinding Behavior of Carbohydrate-Binding Modules

This paper uses acoustic force spectroscopy to study how carbohydrate-binding modules (CBMs) unbind from nanocellulose surfaces, revealing distinct binding conformations and their impact on cellulolytic activity.

Multiscale Characterization of Complex Binding Interactions of Cellulolytic Enzymes Highlights Limitations of Classical Approaches

This study uses single-molecule assays and simulations to show how CBM1 binding affects cellulase activity on different cellulose allomorphs, highlighting limitations of classical binding models.

Cathepsin K maintains the number of lymphocytes in vivo

This study shows that Cathepsin K is essential for maintaining lymphocyte numbers, especially T cells in the bone marrow, but not for early hematopoiesis.