Cell BiologybioRxiv
Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection
V. M. Sluch, X. Chamling, C. Wenger…·D. J. Zack
This paper introduces a method for efficient scarless gene knock-in into human and mouse pluripotent stem cells using transient antibiotic selection, achieving up to 65% efficiency for single reporter genes.