W. Rut

SARS-CoV-2 Mpro protease variants of concern display altered viral and host target processing but retain potency towards antivirals

The paper examines how SARS-CoV-2 Mpro variants affect viral and host protein processing but maintain antiviral potency.

Ebselen derivatives are very potent dual inhibitors of SARS-CoV-2 proteases - PLpro and Mpro in in vitro studies

This study identifies ebselen derivatives as potent inhibitors of SARS-CoV-2 proteases PLpro and Mpro in vitro, with some compounds showing greater potency than ebselen itself.

Activity profiling and structures of inhibitor-bound SARS-CoV-2-PLpro protease provides a framework for anti-COVID-19 drug design

This study profiles SARS-CoV-2-PLpro activity and determines inhibitor structures to guide anti-COVID-19 drug design.

Substrate specificity profiling of SARS-CoV-2 main protease enables design of activity-based probes for patient-sample imaging

The paper profiles SARS-CoV-2 main protease substrate specificity to design activity-based probes for imaging Mpro in patient samples.

Engineered unnatural ubiquitin for optimal detection of deubiquitinating enzymes

The paper designs and tests engineered unnatural ubiquitin substrates and probes to improve selectivity for deubiquitinating enzymes.

The Activome: multiplexed probing of activity of proteolytic enzymes using mass cytometry-compatible activity-based probes (TOF-probes)

The paper develops lanthanide-labeled activity-based probes for mass cytometry to multiplex protease activity detection, demonstrating their use in identifying protease activomes in cell lines and blood cells.