Klas Hatje

This study compares single-cell RNA-seq methods for neutrophil transcriptome profiling in clinical samples, finding all methods produce high-quality data with 10X FLEX showing strong concordance with flow cytometry.

The paper identifies two compounds, trifluoperazine and deoxytubercidin, as novel microglial phagocytosis inhibitors using high-throughput screening and in vivo validation.

Besca is a toolkit that streamlines scRNA-seq analysis with quality control, clustering, cell annotation, and bulk transcriptomics integration across datasets.

The paper evaluates coiled-coil prediction tools using PDB data, finding significant variation in predictions and limited applicability for large datasets.

The paper identifies the origin of a novel exocytic transport mechanism in early animal evolution, tracing SPIRE function to choanoflagellates and showing its role in coordinating actin/myosin forces for cellular communication.

The paper estimates the extent and biological role of mutually exclusive splicing in humans by identifying and validating over 855 MXEs, including novel exons, and links them to disease pathology.