Carlos E. Milla

The paper develops and optimizes two protocols for purifying ssDNA from filamentous phages using chaotropic salts and silica columns, achieving high recovery and purity comparable to commercial kits.

This study compares genome features of Pseudomonas aeruginosa isolates from cystic fibrosis patients and environmental sources, finding smaller genomes and shorter prophage sequences in clinical isolates.

This study identifies a novel mechanism by which Pseudomonas aeruginosa acquires iron through a partnership with filamentous phages, enhancing bacterial growth and pathogenesis.

This study analyzes 186 Pseudomonas aeruginosa clinical isolates to assess the association between prophage presence and antibiotic resistance, finding few significant links.

This study demonstrates that gene-corrected human airway stem cells can durably engraft and restore CFTR function in murine respiratory epithelium, offering a potential therapy for cystic fibrosis.

The paper investigates Pf phage transmission in Pseudomonas aeruginosa airway infections in cystic fibrosis patients, finding vertical transmission within lineages and new superinfections by Pf+ strains.

This paper develops broad-spectrum bacteriophage therapy cocktails effective against multiple bacterial strains and infections.

This study shows Pf phages and sputum polymers reduce antibiotic diffusion through electrostatic binding and liquid crystal formation in chronic Pseudomonas aeruginosa infections.

The paper identifies bacterial receptor genes determining phage host range, showing defense mechanisms are not predictive of susceptibility.

This paper corrects the ΔF508 mutation in CF patient airway stem cells using CRISPR-Cas9 and AAV6, achieving high correction rates and functional rescue in differentiated epithelia.

The paper develops a gene-specific meta-predictor for variant pathogenicity using a Random Forest model trained on the CFTR gene and evaluates its performance against existing tools.