Denis V. Titov

This study identifies purine nucleotides as molecular glues that regulate purine biosynthesis by tethering PPAT to NUDT5, and shows how thiopurine drugs function as metabolic glues with enhanced potency.

The paper shows F26BP regulates glycolytic rate independently of energy state, distinguishing it from other regulators, and identifies downstream intermediates that control F26BP levels.

The paper estimates ATP costs for mammalian cell growth and maintenance, showing that respiration alone cannot meet demands at fast growth rates, necessitating glycolytic ATP production.

The paper develops ATPGobble, a genetically encoded tool to manipulate ATP/ADP ratios in human cells, showing it increases energy demand and activates AMPK.

The paper uses SRS microscopy to show caloric restriction extends C. elegans lifespan by inhibiting vitellogenesis, a process linked to protein buildup in the pseudocoelom.

The paper shows glycolysis produces ATP faster per gram of protein than respiration in E. coli, yeast, and mammalian cells, explaining the Warburg Effect as an optimization of energy production rate under proteome constraints.

The paper develops a genetically encoded tool to manipulate mitochondrial membrane potential and shows it drives the integrated stress response from ATP synthase dysfunction.