Ulrich Rothbauer

This paper introduces NanoBlocks, a method to convert affinity binders into fluorescent biosensors by adding competitive peptides, demonstrating their use in detecting targets in vitro and in cells.

The paper visualizes β-catenin localization during Xenopus nephron development and finds that it becomes enriched at epithelial junctions while remaining in nuclear and cytoplasmic compartments during maturation.

This study uses single-molecule tracking to show that Lrp6 and Ror2 co-assemble into hybrid Wnt signalosomes dynamically.

This paper develops nanobodies targeting hOX40 for in vivo imaging of activated T cells, showing they bind specifically and function as imaging probes without affecting T cell viability.

This study develops nanobodies to monitor Drp1, a mitochondrial fission factor, using proteomics, microscopy, and live-cell imaging techniques to study its dynamics in disease models.

The paper develops a PNGase Rc column for online deglycosylation during HDX-MS, enabling efficient analysis of glycoproteins and mapping of mAb binding epitopes.

This study develops human SIRPα nanobodies for both functional modulation of myeloid cells and in vivo imaging of tumor infiltration.