Dipali G. Sashital

This study investigates how crRNA seed sequence mismatches affect interference and priming in the type I-E CRISPR-Cas system, finding that specific mismatches cause significant defects in immune function.

This study shows that Cas9 nicking of the target DNA strand can provide sufficient phage resistance, while non-target nicking does not enhance protection beyond Cas9 binding.

This study shows that Cas12a's specificity changes with Mg2+ concentration, affecting cleavage efficiency and phage escape routes in different cellular environments.