Tessa Van de Steene

This study expands a modular cloning platform to enable rapid, customizable construct assembly for MAPPIT assays, demonstrating its application in studying multiple protein-protein interactions.

This study identifies SopB-associated host proteins using Virotrap, revealing SopB's interaction with ESCRT-0 and its role in Salmonella's manipulation of host endosomal trafficking.

This paper introduces a 96-well ultrafiltration method for isolating extracellular vesicles from conditioned medium, improving purity and reproducibility for proteomic analysis.

The paper introduces a T2A split-link system for proximity labeling proteomics to improve control over enzymatic activity and expression levels, enhancing accuracy in identifying protein interactions.

The paper investigates N-terminal proteoforms and finds they can engage in different protein complexes compared to their canonical counterparts.

The paper investigates non-coding transcript translation in HEK293T cells, finding limited but potentially functional translation products using ribosome profiling and mass spectrometry.