Javier Rey-Barroso

The paper uses cryo-electron tomography and computational methods to infer the assembly states of actin networks in podosomes, revealing layered helical order and preferred orientation transitions.

The paper introduces 4polar3D, a method for 3D orientation imaging of dense actin networks using ratiometric polarization splitting, demonstrating its ability to resolve nanoscale organization in cellular structures.

This study shows that ezrin, radixin, and moesin are not required for macrophage migration or actin cortex mechanics, challenging their universal importance in these processes.